ydaK [2017-02-27 15:02:04]

general stress protein, may act as c-di-GMP receptor, required for extracellular polysaccharide synthesis by YdaL-YdaM-YdaN

locus: BSU04280

pI: 6.00

mw: 32.00 kDa

protein length: 283 aa

gene length: 849 bp

function: unknown

product: general stress protein

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.2|Targets of c-di-GMP]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

This gene is a member of the following [SW|regulons]

• [SW|SigB regulon]

Gene

Coordinates on the chromosome (coding sequence): 480,013 -> 480,864

The protein

[SW|Domains]

• contains a C-terminal degenerated [SW|GGDEF domain] [Pubmed|23893111]

[SW|Cofactors]

• binds c-di-GPM at high concentration (K(D) for c-di-GMP: 1.1 myM) [Pubmed|23893111]

[SW|Localization]

• cell membrane, forms clusters at the cell poles and septa (with [protein|961538BEDD27C24FC073AFB7AB3268E3D46783CC|YdaM] and [protein|ADE0122D8FFF2F78B6D21031B7D02A323A13ED63|YdaN]) [Pubmed|27897378]

Expression and Regulation: [[this]]

Biological materials

Mutant

• MGNA-C074 (ydaK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2072 NBRP B. subtilis, Japan]
• GP1592 (kan) available in [SW|Jörg Stülke]'s lab

References

• 22383849,22821967,23893111,27897378